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Proceedings Paper

Fiber optic immunosensors to monitor small-molecule analytes in groundwater
Author(s): Jeffrey T. Ives; Heide M. Doss; Brian J. Sullivan; John C. Stires; James H. Bechtel
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Paper Abstract

Analytical characterization of environmental sites has become a DLR1 to DLR2 billion dollar industry, and field methods to provide lower cost, more rapid analyses are being developed and commercialized. Antibody kits and fieldable gas chromatographs are examples of this work, but they still require extensive manual sample collection and preparation. As a further advance, we are developing a fieldable environmental monitor based on antibodies, fiber optic probes, and compact optoelectronics. Antibodies are immobilized to the surface of etched optical fiber tips, and a displacement reaction between the target molecules and fluor-labeled target analogs is monitored remotely. For extended, autonomous operation, fluor-labeled reagents are contained within a size-selective membrane that surrounds the fiber tip. This creates a small probe head that easily fits within a standard 2' diameter groundwater well. A hand- sized instrument with laser diode, 20-bit A/D, 12 MHz microcontroller, and associated components has also been assembled. Detection limits of 1 ppb for BTEX compounds (benzene, toluene, ethylbenzene, xylene) have been measured. Molecular imprints as lower cost and potentially most durable biomimetic analogs to the antibodies are also being investigated. Preliminary results with toluene and trichloroethylene targets have been obtained with molecularly imprinted fiber optic sensors.

Paper Details

Date Published: 23 February 1999
PDF: 9 pages
Proc. SPIE 3540, Chemical, Biochemical, and Environmental Fiber Sensors X, (23 February 1999); doi: 10.1117/12.339806
Show Author Affiliations
Jeffrey T. Ives, TACAN Corp. (United States)
Heide M. Doss, TACAN Corp. (United States)
Brian J. Sullivan, TACAN Corp. (United States)
John C. Stires, TACAN Corp. (United States)
James H. Bechtel, TACAN Corp. (United States)

Published in SPIE Proceedings Vol. 3540:
Chemical, Biochemical, and Environmental Fiber Sensors X
Robert A. Lieberman, Editor(s)

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