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Proceedings Paper

Formation of protoporphyrin IX in mouse skin after topical application of 5-aminolevulinic acid and its methyl esther
Author(s): Roar Sorensen; Petras Juzenas; Vladimir Iani; Johan Moan
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Paper Abstract

Normal skin of nude mice (Balb/c) was treated topically with 5-aminolevulinic acid (ALA) and its methyl ester (ALA-Me) for 24 hours. Approximately 0.1 gram of freshly prepared cream was applied to a spot of 1 cm2 on the flank of the mice, which was then covered with a transparent dressing. The ALA induced protoporphyrin IX (PpIX) was studied by means of a noninvasive fiber-optic fluorescence probe connected to a luminescence spectrometer. The excitation wavelength was 407 nm, and the emission wavelength was 637 nm. For the first hour a slight lag in PpIX production was observed for the mice treated with ALA-Me compared to the mice treated with ALA. After approximately 12 hours the ALA and the ALA-Me treated mice showed the same PpIX fluorescence intensity. From 12 hours until 24 hours the PpIX fluorescence intensity decreased for both treatment modalities, even though ALA and ALA-Me were continuously present. At 24 hours ALA-Me-treated mice had less than half the amount of PpIX in their skin compared with ALA- treated mice.

Paper Details

Date Published: 3 February 1999
PDF: 5 pages
Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); doi: 10.1117/12.339121
Show Author Affiliations
Roar Sorensen, The Norwegian Radium Hospital (Norway)
Petras Juzenas, Vilnius Univ. (Norway)
Vladimir Iani, The Norwegian Radium Hospital (Norway)
Johan Moan, The Norwegian Radium Hospital (Norway)

Published in SPIE Proceedings Vol. 3563:
Photochemotherapy of Cancer and Other Diseases
Benjamin Ehrenberg; Kristian Berg, Editor(s)

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