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Proceedings Paper

Microbial analysis using Sharma's reaction
Author(s): Zulfiqur Ali; Bruce Hodson; James Timmins; Stephen Keith
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Paper Abstract

The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallin. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallin in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.

Paper Details

Date Published: 10 February 1999
PDF: 6 pages
Proc. SPIE 3534, Environmental Monitoring and Remediation Technologies, (10 February 1999); doi: 10.1117/12.339046
Show Author Affiliations
Zulfiqur Ali, Univ. of Teesside (United Kingdom)
Bruce Hodson, Univ. of Teesside (United Kingdom)
James Timmins, Univ. of Teesside (United Kingdom)
Stephen Keith, Univ. of Teesside (United Kingdom)

Published in SPIE Proceedings Vol. 3534:
Environmental Monitoring and Remediation Technologies
Tuan Vo-Dinh; Robert L. Spellicy, Editor(s)

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