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Proceedings Paper

Two-dimensional fluorescence spectroscopy for application in biotechnology
Author(s): Carsten Lindemann; S. Marose; Thomas-Helmut Scheper; Hans Ole Nielsen; Bernd Hitzmann; K.-H. Belgardt
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Paper Abstract

A wide range of excitation and emission wavelengths is measured using the technique of two-dimensional (2D-) fluorescence spectroscopy. In a single, so called, two- dimensional fluorescence spectrum several biogenic fluorophors like proteins, vitamins and coenzymes can be detected simultaneously. This can give important information for bioprocess monitoring and control. An optical sensor (BioViewR) for on line fluorescence measurements at industrial (bio)-processes was used to get the results presented in this paper. This BioViewR-sensor is optimized to work in the harsh environment of production sites in biotechnological industry and -- using an optical light guide system with open-end detection -- it is very well suited for in vivo measurements, because it is non-invasive and the on line data can be performed in-situ.

Paper Details

Date Published: 10 February 1999
PDF: 8 pages
Proc. SPIE 3534, Environmental Monitoring and Remediation Technologies, (10 February 1999); doi: 10.1117/12.338986
Show Author Affiliations
Carsten Lindemann, Univ. of Hannover and DELTA Light and Optics (Denmark)
S. Marose, Univ. of Hannover (Germany)
Thomas-Helmut Scheper, Univ. of Hannover (Germany)
Hans Ole Nielsen, DELTA Light&Optics (Denmark)
Bernd Hitzmann, Univ. of Hannover (Germany)
K.-H. Belgardt, Univ. of Hannover (Germany)

Published in SPIE Proceedings Vol. 3534:
Environmental Monitoring and Remediation Technologies
Tuan Vo-Dinh; Robert L. Spellicy, Editor(s)

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