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Proceedings Paper

Concentration, size, and excitation power effects on fluorescence from microdroplets and microparticles containing tryptophan and bacteria
Author(s): Nicholas F. Fell Jr.; Ronald G. Pinnick; Steven C. Hill; Gorden W. Videen; Stanley Niles; Richard K. Chang; Stephen Holler; Yongle Pan; Jerold R. Bottiger; Burt V. Bronk
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Paper Abstract

Our group has been developing a system for single-particle fluorescence detection of aerosolized agents. This paper describes the most recent steps in the evolution of this system. The effects of fluorophore concentrations, droplet size, and excitation power have also been investigated with microdroplets containing tryptophan in water to determine the effects of these parameters on our previous results. The vibrating orifice droplet generator was chosen for this study base don its ability to generate particles of well- known and reproducible size. The power levels required to reach saturation and photodegradation were determined. In addition, the collection of fluorescence emission was optimized through the use of a UV achromatic photographic lens. This arrangement permitted collection of images of the droplet stream. Finally, the use of a dual-beam, conditional firing scheme facilitated the collection of improved signal- to-noise single-shot spectra from individual biological particles.

Paper Details

Date Published: 18 January 1999
PDF: 12 pages
Proc. SPIE 3533, Air Monitoring and Detection of Chemical and Biological Agents, (18 January 1999); doi: 10.1117/12.336843
Show Author Affiliations
Nicholas F. Fell Jr., Army Research Lab. and New Mexico State Univ. (United States)
Ronald G. Pinnick, Army Research Lab. (United States)
Steven C. Hill, Army Research Lab. (United States)
Gorden W. Videen, Army Research Lab. (United States)
Stanley Niles, Army Research Lab. (United States)
Richard K. Chang, Yale Univ. (United States)
Stephen Holler, Yale Univ. (United States)
Yongle Pan, Yale Univ. (United States)
Jerold R. Bottiger, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
Burt V. Bronk, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 3533:
Air Monitoring and Detection of Chemical and Biological Agents
Joseph Leonelli; Mark L.G. Althouse, Editor(s)

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