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Proceedings Paper

Optical tomography of living cells using a phase-shifting Linnik microscope
Author(s): Gennady N. Vishnyakov; Gennady G. Levin
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Paper Abstract

To obtain three-dimensional refractive index or density spatial distribution inside optically transparent living cells, it is necessary to combine computed tomography and interference microscopy. These methods use the oblique illumination and the sample viewing angles scanning for recording a number of the projections. A new application Linnik microscope reflected type for obtaining a high quality phase projections with spatially incoherent laser illumination is described. Phase-shifting technique for automated interferogram analysis is used. Interferometric computed- tomography Linnik laser microscope are fully automated integrated systems that incorporate CCD camera, frame grabber, computed operated PZT mirror as well as intelligible software for interferogram processing, projections preprocessing and tomographic reconstruction. Microscope measurement system and performance characterization are presented. There are some features in angle scanning, projections proceeding, and reconstruction for tomographic investigation of the phase samples that are placed near the mirror surface. For plane mirror the projections viewing angle range may be limited to 90-degree and the investigation object may be represented as a sum of the original object and its mirror reflection. The iterative algorithms for limited-angle tomographic reconstruction were used. Three-dimensional images of living blood cells (lymphocytes) were obtained.

Paper Details

Date Published: 19 January 1999
PDF: 4 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336834
Show Author Affiliations
Gennady N. Vishnyakov, Russian Research Institute for Optophysical Measurements (Russia)
Gennady G. Levin, Russian Research Institute for Optophysical Measurements (Russia)

Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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