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Proceedings Paper

Localization of near-infrared contrast agents in tumors by intravital microscopy
Author(s): Andreas Becker; Guenther Schneider; Bjoern Riefke; Kai Licha; Wolfhard Semmler
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Paper Abstract

In this contribution we use intravital microscopy to study the dynamics of extravasation into normal and tumor tissue of several hydrophilic cyanine dyes used as near-infrared (NIR) contrast agents. The technique provides information about the angiographic properties of the dyes and about their interaction with tumor tissue under dynamic conditions in vivo. In our previous work we demonstrated that several NIR- absorbing fluorescent dyes enable in vivo fluorescence detection of tumors in mice and rats. However, the mechanism leading to dye accumulation and enhanced fluorescence in tumors is not fully understood. Increased extravasation of dyes into tumor tissue due to pathologically altered tumor vessels may be an important factor in this process. Indocyanine green (ICG) displayed predominantly intravascular distribution and rapid elimination resulting in enhanced fluorescence signal of vessels during the first 15 min after administration only. No elevated extravasation into tumor tissue was observed with ICG. A hydrophilic indotricarbocyanine derivative with a high molecular weight displayed prolonged intravascular distribution and increased fluorescence signal of the vasculature compared to surrounding tissue for up to five hours. Rapid extravasation and accumulation in tumor areas, yielding elevated contrast of tumors up to 15 min after administration, was observed with hydrophilic, low molecular weight indotricarbocyanine derivatives.

Paper Details

Date Published: 19 January 1999
PDF: 7 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336824
Show Author Affiliations
Andreas Becker, Institut fuer Diagnostikforschung ad der Freie Univ. Berlin (Germany)
Guenther Schneider, Universitaetskliniken des Saarlandes (Germany)
Bjoern Riefke, Institut fuer Diagnostikforschung ad der Freie Univ. Berlin (Germany)
Kai Licha, Institut fuer Diagnostikforschung an der Freie Univ. Berlin (Germany)
Wolfhard Semmler, Institut fuer Diagnostikforshung an derFreie Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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