The infrared spectrum of biological tissue is due to the 'microheterogenous' composition as a whole and is based on complex vibrational modes. In detail, the spectrum represents the biochemical status resulting from a combination of the structural framework of tissues together with the biological active compounds of metabolism. According to the literature, the main IR-spectroscopic differences are to be expected by the characteristic spectral pattern which is located at the 'fingerprint' region (1500 - 1000 cm^-1). In order to design and develop an endoscopic system for the in vivo identification of healthy and malignant tissue FTIR- measurements were carried out using a fiberoptic device. The source of IR-radiation can be either the FTIR-spectrometer or tunable IR-diode lasers. Fiberoptic IR-spectra obtained using the attenuated total reflectance (ATR) and reflection mode were compared to spectra resulting from the FTIR- microspectroscopic measurements.

Date Published: 19 January 1999
PDF: 8 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336815

Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)