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Proceedings Paper

High-speed separation using miniaturized slab gel and high-spatial-resolution detection by a thermal lens microscope
Author(s): Tamao Odake; Jinjian Zheng; Takehiko Kitamori; Tsuguo Sawada
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Paper Abstract

Slab gel electrophoresis is a major tool for size-separation of DNA fragments and proteins. However, conventional slab gel electrophoresis uses a large gel to obtain sufficient separation and it takes several hours to separate, because conventional detectors using radio isotopes and fluorescence are affected by the strongly light-scattering gel and do not have enough spatial resolution to be applied to a miniaturized gel. Thermal lens microscope (TLM), which we have developed, has spatial resolution of micrometer level, and is not easily affected by the scattered light and can maintain high spatial resolution. We demonstrate here high- speed separation using a miniaturized gel and successive high spatial resolution detection using TLM. Using a miniaturized gel one-tenth of conventional gel in size, DNA fragments (100 base pair ladder sample) were separated in 5 to 15 minutes, which was about one order of magnitude faster than using a conventional gel, and successfully detected by TLM.

Paper Details

Date Published: 4 January 1999
PDF: 8 pages
Proc. SPIE 3565, Thermal Therapy, Laser Welding, and Tissue Interaction, (4 January 1999); doi: 10.1117/12.335793
Show Author Affiliations
Tamao Odake, Kanagawa Academy of Science and Technology (Japan)
Jinjian Zheng, Univ. of Tokyo (Japan)
Takehiko Kitamori, Kanagawa Academy of Science and Technology (Japan)
Tsuguo Sawada, Univ. of Tokyo (Japan)

Published in SPIE Proceedings Vol. 3565:
Thermal Therapy, Laser Welding, and Tissue Interaction
Stephen G. Bown; Guy P. Delacretaz; Guilhem Godlewski M.D.; Gerhard J. Mueller; Roberto Pini; Hans-Dieter Reidenbach; Rudolf W. Steiner; Lars Othar Svaasand; Karl-Goran Tranberg, Editor(s)

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