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Proceedings Paper

Detection of bacteria from a cecal anaerobic competitive exclusion culture with an immunoassay electrochemiluminescence sensor
Author(s): Ross C. Beier; Colin R. Young; Larry H. Stanker
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Paper Abstract

A competitive exclusion (CE) culture of chicken cecal anaerobes has been developed and used in this laboratory for control of Salmonella typhimurium in chickens. The CE culture consists of 29 different species of micro-organisms, and is known as CF3. Detection of one of the CF3 bacteria, Eubacteria, and S. typhimurium were demonstrated using a commercial immunomagnetic (IM) electrochemiluminescence (ECL) sensor, the ORIGENR Analyzer. Analysis was achieved using a sandwich immunoassay. Bacteria were captured on antibody- conjugated 280 micron sized magnetic beads followed by binding of reporter antibodies labelled with ruthenium (II) tris(dipyridyl) chelate [Ru(bpy)32+]. The magnetic beads were then trapped on an electrode in the reaction cell of the ORIGENR Analyzer by a magnet, and the ECL was evoked from Ru(bpy)32+ on the tagged reporter antibodies by an electrical potential at the electrode. Preliminary IM-ECL assays with Eubacteria yielded a detection limit of 105 cfu/mL. Preliminary IM-ECL assays with S. typhimurium yielded a similar detection limit of 105 cfu/mL.

Paper Details

Date Published: 12 January 1999
PDF: 11 pages
Proc. SPIE 3544, Pathogen Detection and Remediation for Safe Eating, (12 January 1999); doi: 10.1117/12.335773
Show Author Affiliations
Ross C. Beier, USDA Agricultural Research Service (United States)
Colin R. Young, USDA Agricultural Research Service (United States)
Larry H. Stanker, USDA Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 3544:
Pathogen Detection and Remediation for Safe Eating
Yud-Ren Chen, Editor(s)

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