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Proceedings Paper

Waveguide immunofluorescence sensor for water pollution analysis
Author(s): Richard D. Harris; G. R. Quigley; James S. Wilkinson; Albrecht Klotz; C. Barzen; Andreas Brecht; Guenter Gauglitz; R. A. Abuknesha
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Paper Abstract

A regenerable channel waveguide fluorescence sensor for environmental monitoring is reported. The sensor has been characterized as a detector of the pesticide 2,4 dichlorophenoxyacetic acid. A binding inhibition assay, using fluorescent Cy5.5 dye-labeled antibodies, was monitored at the modified surface of the glass waveguide to detect the target analyte. Three calibration curves were determined and averaged. The averaged calibration curve has a mid-point of 0.68 ppb and a calculated detection limit of 0.28 ppb. Incorporation of a 20 nm thick tantalum pentoxide film at the waveguide surface enhanced the peak fluorescence signal by a factor of approximately 6 compared with an uncoated sensor.

Paper Details

Date Published: 18 December 1998
PDF: 9 pages
Proc. SPIE 3539, Chemical Microsensors and Applications, (18 December 1998); doi: 10.1117/12.333751
Show Author Affiliations
Richard D. Harris, Univ. of Southampton (United Kingdom)
G. R. Quigley, Univ. of Southampton (United Kingdom)
James S. Wilkinson, Univ. of Southampton (United Kingdom)
Albrecht Klotz, Eberhard-Karls-Univ. Tuebingen (Germany)
C. Barzen, Eberhard-Karls-Univ. Tuebingen (Germany)
Andreas Brecht, Eberhard-Karls-Univ. Tuebingen (Germany)
Guenter Gauglitz, Eberhard-Karls-Univ. Tuebingen (Germany)
R. A. Abuknesha, GEC-Marconi Materials Technology Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 3539:
Chemical Microsensors and Applications
Stephanus Buettgenbach, Editor(s)

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