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Proceedings Paper

Release of thromboxane by photodynamically activated rat platelets
Author(s): Chuannong Zhou; Geng-Hong Zhou; Jinsheng Deng; Junlin Liang; Wen-Kai Liu
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Paper Abstract

The purpose of this study is to explore the significance of photodynamic activation of platelets in the mechanism of photodynamic destruction of tumors. Normal rats were divided into control and experimental groups, 6 rats in each group. Hematoporphyrin derivative was given in a dose of 10 or 20 mg/kg iv. Blood was taken and plasma rich in platelets was obtained by centrifugation. HeNe laser irradiation of the platelets in plasma was performed ex vivo at 30 mW, spot diameter of 6 mm for 16 or 32 minutes (100 or 200 J/cm2). Aspirin was given in one group in a dose of20 mg/kg p.o.at 24 and 3hrs prior to irradiation, while indomethacin was given in another group in a dose of 0.5 mg/kg. The mean± standard diviation of TXB2 and 6-keto-PGF-lcxconcentration in the plasma, and the TXB2/6-keto-PGF-la ratio were calculated for each group and compared statistically between groups by Student's t-test. The results showed that the TXA2 release was tending to increase and TXB2/6-keto-PGF-la ratio was significantly increased after PDT treatment in a dose-response relationship. Our findings evidently support that platelet activation may play an important role in the mechanism of microvascular damage, intravascular clotting and microcirculatory disturbance induced by photodynamic therapy. Indomethacin showed a more strong inhibitory effect on this process than aspirin did. It indicates that in patients taking indomethacin or aspirin, photodynamic therapy should be performed a few day after stopping those drugs. Key words: Photodynamic therapy, Platelets, Thromboxanes, Prostacyclins

Paper Details

Date Published: 2 November 1998
PDF: 7 pages
Proc. SPIE 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery, (2 November 1998); doi: 10.1117/12.330175
Show Author Affiliations
Chuannong Zhou, Cancer Institute (China) and Peking Union Medical College (China)
Geng-Hong Zhou, Cancer Institute (China) and Peking Union Medical College (China)
Jinsheng Deng, Cancer Institute (China) and Peking Union Medical College (China)
Junlin Liang, Cancer Institute (China) and Peking Union Medical College (China)
Wen-Kai Liu, Cancer Institute (China) and Peking Union Medical College (China)


Published in SPIE Proceedings Vol. 3344:
1997 Shanghai International Conference on Laser Medicine and Surgery
Jing Zhu, Editor(s)

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