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Proceedings Paper

PDT mechanism of phthalocyanines by ultrastructural cytochemical cerium deposition localization EDX technique and apoptosis detection
Author(s): Hong-Yu Yu; Yong-Ji Yang; Ji-Hong Sha; Rong-Chun Dong; Cheng-Zhi Xiao; Fa-Pu Chen
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Paper Abstract

Both the chemical mechanisms of photodynamic therapy (PDT) with new photosensitizer phthalocyanine (PC) and the relationship between the chemical mechanisms and the pathologic mechanism in PDT with PC (PC PDT) are not exactly clear. In this study, the mechanisms of PC-PDT in vitro and in vivo were aimed with research methods of ultrastructural cytochemical cerium deposition locations, EDX technique and apoptosis detection. Three kinds of PCs, disulfonated chloroaluminium PC, AlS3PC and disulfonated zinc PC, were adopted in the experiments. The result revealed: (1) There are a direct proportion between the amount of the 1O2 enhancer and the amount of cerium deposition and an inverse ratio between the amount of the 1O2 quencher and the deposition. So the dominant mechanism of PC-PDT is 1O2; (2) The ultrastructural sties of most obvious pathologic damage ar the sites of richness in PC and the sites of richness in cerium deposition; the mitochondria is one of the most important PDT targets in tumor cells, and secondly other cytomembrane structures are also important sites injured by PC-PDT; both the PDT damages to tumor cells and that to microvasculature are the significant pathologic mechanisms for killing tumor cells in vivo; (3) Apoptosis is an event in the tumor killing process of PC-PDT.

Paper Details

Date Published: 2 November 1998
PDF: 5 pages
Proc. SPIE 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery, (2 November 1998); doi: 10.1117/12.330164
Show Author Affiliations
Hong-Yu Yu, Second Military Medical Univ. (China)
Yong-Ji Yang, Second Military Medical Univ. (China)
Ji-Hong Sha, Second Military Medical Univ. (China)
Rong-Chun Dong, Second Military Medical Univ. (China)
Cheng-Zhi Xiao, Second Military Medical Univ. (China)
Fa-Pu Chen, Second Military Medical Univ. (China)

Published in SPIE Proceedings Vol. 3344:
1997 Shanghai International Conference on Laser Medicine and Surgery
Jing Zhu, Editor(s)

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