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Proceedings Paper

Video-rate spectral imaging of fluorescence phonomena
Author(s): Bridget K. Ford; Curtis Earl Volin; Michael R. Descour; John Phillips Garcia; Daniel W. Wilson; Paul D. Maker; Gregory H. Bearman
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Paper Abstract

We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer ((mu) CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, the (mu) CTIS can be used to image dynamic phenomena involving multiple, spectrally overlapping fluorescence probes. The result presented in this paper consists of proof-of-concept imaging result using two static targets. The first is composed of 6-micrometers fluorescing microspheres and the second consists of rat sinusoid epithelial cells loaded with 0.5-micrometers fluorescing microspheres. Image data were collected in integration times of 16 msec, comparable to video frame rate integration times. The emission spectra were sampled at 10-nm intervals between 420 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 micrometers .

Paper Details

Date Published: 16 October 1998
PDF: 8 pages
Proc. SPIE 3438, Imaging Spectrometry IV, (16 October 1998); doi: 10.1117/12.328119
Show Author Affiliations
Bridget K. Ford, Optical Sciences Ctr./Univ. of Arizona (United States)
Curtis Earl Volin, Optical Sciences Ctr./Univ. of Arizona (United States)
Michael R. Descour, Optical Sciences Ctr./Univ. of Arizona (United States)
John Phillips Garcia, Optical Sciences Ctr./Univ. of Arizona (United States)
Daniel W. Wilson, Jet Propulsion Lab. (United States)
Paul D. Maker, Jet Propulsion Lab. (United States)
Gregory H. Bearman, Jet Propulsion Lab. (United States)

Published in SPIE Proceedings Vol. 3438:
Imaging Spectrometry IV
Michael R. Descour; Sylvia S. Shen, Editor(s)

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