Share Email Print

Proceedings Paper

Extraordinary survival of nanobacteria under extreme conditions
Author(s): Michael Bjorklund; Neva Ciftcioglu; E. Olavi Kajander
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Nanobacteria show high resistance to gamma irradiation. To further examine their survival in extreme conditions several disinfecting and sterilizing chemicals as well as autoclaving, UV light, microwaves, heating and drying treatments were carried out. The effect of antibiotics used in cell culture were also evaluated. Two forms of nanobacteria were used in the tests: nanobacteria cultured in serum containing medium, and nanobacteria cultured in serum-free medium, the latter being more mineralized. Nanobacteria, having various amounts of apatite on their surfaces, were used to analyze the degree of protection given by the mineral. The chemicals tested included ethanol, glutaraldehyde, formalin, hypochlorite, hydrogen peroxide, hydrochloric acid, sodium hydroxide, detergents, and commercial disinfectants at concentrations generally used for disinfection. After chemical and physical treatments for various times, the nanobacteria were subcultered to detect their survival. The results show unique and wide resistance of nanobacteria to common agents used in disinfection. It can also be seen that the mineralization of the nanobacterial surface furthermore increases the resistance. Survival of nanobacteria is unique among living bacteria, but it can be compared with that observed in spores. Interestingly, nanobacteria have metabolic rate as slow as bacterial spores. A slow metabolic rate and protective structures, like mineral, biofilm and impermeable cell wall, can thus explain the observations made.

Paper Details

Date Published: 6 July 1998
PDF: 7 pages
Proc. SPIE 3441, Instruments, Methods, and Missions for Astrobiology, (6 July 1998); doi: 10.1117/12.319827
Show Author Affiliations
Michael Bjorklund, Univ. of Kuopio (Finland)
Neva Ciftcioglu, Univ. of Kuopio (Finland)
E. Olavi Kajander, Univ. of Kuopio (Finland)

Published in SPIE Proceedings Vol. 3441:
Instruments, Methods, and Missions for Astrobiology
Richard B. Hoover, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?