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Proceedings Paper

3D microscopy with phase-shifting digital holography
Author(s): Tong Zhang; Ichirou Yamaguchi
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Paper Abstract

In this paper, we applied phase-shifting digital holography to microscopy. The digital holograms are recorded by a video camera from the superposition of a plane reference beam and the object beam passed through a microscopic objective. The distribution of complex amplitude of the object wave is derived by using the phase-shifting algorithm and then Fresnel transformed in a computer to reconstruct images at arbitrary planes. After relationships between object position and position of focused image and magnification are discussed, those are applied to determine the sizes and positions of small particles by image processing technique.

Paper Details

Date Published: 6 July 1998
PDF: 8 pages
Proc. SPIE 3479, Laser Interferometry IX: Applications, (6 July 1998); doi: 10.1117/12.316445
Show Author Affiliations
Tong Zhang, Saitama Univ. (Japan)
Ichirou Yamaguchi, RIKEN--The Institute of Physical and Chemical Research (Japan) and Saitama Univ. (Japan)

Published in SPIE Proceedings Vol. 3479:
Laser Interferometry IX: Applications
Ryszard J. Pryputniewicz; Gordon M. Brown; Werner P. O. Jueptner, Editor(s)

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