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Proceedings Paper

Laser-induced saturation of fluorescence for complex organic molecules
Author(s): Victor I. Yuzhakov; K. G. Yevsyukhina; Svetlana V. Patsayeva
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Paper Abstract

The effect of fluorescence saturation is caused by limited life-time of molecules in excited state, and appears as a non-linear dependence of detected fluorescence signal versus excitation intensity under laser pulsed irradiation of the sample. It is noticeable at conditions of 1024 exciting photons per second per square centimeter, which we usually have for laboratory laser fluorimeter. Spectroscopy of saturated fluorescence is a promising tool for studying such spectral molecular characteristics, as fluorescence life- time, absorption cross-section, constant of singlet-singlet annihilation, and their changes due to molecular interaction. The work presents the theory of the effect considering the cases of different intermolecular interaction (quenching mechanisms, singlet-singlet annihilation) and describes the experimental results on florescence saturation for several organic dyes in water.

Paper Details

Date Published: 26 May 1998
PDF: 9 pages
Proc. SPIE 3404, ALT'97 International Conference on Laser Surface Processing, (26 May 1998); doi: 10.1117/12.308644
Show Author Affiliations
Victor I. Yuzhakov, Moscow State Univ. (Russia)
K. G. Yevsyukhina, Moscow State Univ. (Russia)
Svetlana V. Patsayeva, Moscow State Univ. (Germany)

Published in SPIE Proceedings Vol. 3404:
ALT'97 International Conference on Laser Surface Processing
Vladimir I. Pustovoy, Editor(s)

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