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Proceedings Paper

Effect of antioxidants on PDT treatment of cultured tumor cells
Author(s): Vladislava Melnikova; Lina N. Bezdetnaya; Irina Belitchenko; Alexander Ya. Potapenko; Jean-Louis Merlin; Francois H. Guillemin
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Paper Abstract

Lipid peroxidation (LP) is involved in cell damage induced by photodynamic treatment (PDT) sensitized by some lipophylic porphyrins. We investigated an effect of lipophylic antioxidant (alpha) -tocopherol and its water-soluble analog, trolox, on meta-tetra(hydroxyphenyl)chlorin (mTHPC) sensitized PDT (413 nm) of cultured human colon adenocarcinoma cells (HT29). Cell survival was measured by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide conversion to farmazan (MTT assay). Both antioxidants in concentrations lower than 0.1 mM did not affect photokilling of HT29 cells. These data might suggest that LP is not of crucial importance in cell damage photosensitized by mTHPC. One mM (alpha) -tocopherol or trolox decreased cell survival by ca. 15 and 13% respectively. Both antioxidants increased PDT- induced damage of HT29. Potentiation was evident as the decrease in the initial shoulder part of fluence dependence curve. We propose that antioxidants at height, pro-oxidant concentrations can potentiate PDT induced killing of tumor cells.

Paper Details

Date Published: 19 May 1998
PDF: 5 pages
Proc. SPIE 3247, Optical Methods for Tumor Treatment and Detections: Mechanisms and Techniques in Photodynamic Therapy VII, (19 May 1998); doi: 10.1117/12.308142
Show Author Affiliations
Vladislava Melnikova, Ctr. Alexis Vautrin and Russian State Medical Univ. (Russia)
Lina N. Bezdetnaya, Ctr. Alexis Vautrin (France)
Irina Belitchenko, Ctr. Alexis Vautrin (Russia)
Alexander Ya. Potapenko, Russian State Medical Univ. (Russia)
Jean-Louis Merlin, Ctr. Alexis Vautrin (France)
Francois H. Guillemin, Ctr. Alexis Vautrin (France)

Published in SPIE Proceedings Vol. 3247:
Optical Methods for Tumor Treatment and Detections: Mechanisms and Techniques in Photodynamic Therapy VII
Thomas J. Dougherty, Editor(s)

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