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Proceedings Paper

Light scattering from metal sol labels on high-density DNA probe arrays
Author(s): Mark O. Trulson; Ian D. Walton; Audrey D. Suseno; Hajime Matsuzaki; David Stern
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Paper Abstract

We have been exploring the use of light scattering as a means to detect the binding of nucleic acids to high density DNA probe arrays. Initial work has concentrated on the use of 100 nanometer gold particles conjugated to monoclonal antibodies. A probe array scanner that utilizes an arc lamp source and a `photocopier grade' linear CCD detector has been developed. The optical configuration of the scanner maximizes dynamic range and minimizes optical backgrounds. Initial development of light scattering detection for the p53 cancer gene application shows that functional performance may be obtained that is essentially equivalent to existing fluorescence detection methodology.

Paper Details

Date Published: 10 April 1998
PDF: 7 pages
Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); doi: 10.1117/12.307333
Show Author Affiliations
Mark O. Trulson, Affymetrix, Inc. (United States)
Ian D. Walton, Affymetrix, Inc. (United States)
Audrey D. Suseno, Affymetrix, Inc. (United States)
Hajime Matsuzaki, Affymetrix, Inc. (United States)
David Stern, Affymetrix, Inc. (United States)

Published in SPIE Proceedings Vol. 3259:
Systems and Technologies for Clinical Diagnostics and Drug Discovery
Gerald E. Cohn; John C. Owicki, Editor(s)

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