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Proceedings Paper

Fluorescence-lifetime technologies for high-throughput screening
Author(s): Todd E. French; John C. Owicki; Douglas N. Modlin; Sudhir S. Deshpande; I. Mineyev; Kimberly Crawford; Will Burton
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Paper Abstract

Fluorescence lifetime, the mean interval between absorption and emission, is as fundamental a characteristic of fluorescence as excitation and emission wavelengths and quantum yield. Yet, with the exception of time-resolved fluorescence assays utilizing lanthanide chelates, the analytical possibilities of methods based on fluorescence lifetime are virtually unexploited outside the academic research laboratory. We discuss the potential use of fluorescence-lifetime technologies in high-throughput screening from the standpoint of assay reagents and instrumentation. Among these applications are fluorescence- polarization assays based on long-lifetime probes and fluorescence-intensity assays using lifetime-resolved detection to reject background. We find that fluorescence- lifetime technologies offer significant practical advantages over existing methods.

Paper Details

Date Published: 10 April 1998
PDF: 10 pages
Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); doi: 10.1117/12.307330
Show Author Affiliations
Todd E. French, LJL BioSystems, Inc. (United States)
John C. Owicki, LJL BioSystems, Inc. (United States)
Douglas N. Modlin, LJL BioSystems, Inc. (United States)
Sudhir S. Deshpande, LJL BioSystems, Inc. (United States)
I. Mineyev, LJL BioSystems, Inc. (United States)
Kimberly Crawford, LJL BioSystems, Inc. (United States)
Will Burton, LJL BioSystems, Inc. (United States)

Published in SPIE Proceedings Vol. 3259:
Systems and Technologies for Clinical Diagnostics and Drug Discovery
Gerald E. Cohn; John C. Owicki, Editor(s)

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