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Proceedings Paper

Development of fluorescence-based high-throughput screening assays: choice of appropriate instrumentation
Author(s): David J. Burns; Elisabeth Alder; Yi-Hong Fan; Evelyn McKeegan; Usha Warrior; Bruce Beutel
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Paper Abstract

Fluorescence-based assays have become increasingly popular in high throughput screening for a variety of reasons (e.g. sensitivity). However, new screening technologies are pushing the limits of conventional fluorescence plate readers. For example, instruments that have optical sensitivities beyond most of the commercially available plate readers are required to reproducibly measure the fluorescence generated by the green fluorescent protein (GFP)--a novel reporter gene. Also, miniaturization of screening formats (with densities higher than the conventional 96-well plate) requires high resolution instrumentation to measure fluorescence. Several assays based on optical fluorescence measurements have been developed and screened in our Biological Screening group. These assays include various fluorescence-based protease assays (standard end-point and kinetic modes) and a functional cell-based screen using the green fluorescent protein as a reporter gene. The choice of instrumentation was the critical factor in the performance and success of each of these arrays. Data will be presented for the cell- based reporter assay including the type of instrumentation (fluorescence plate readers; fluorescence imaging systems) used for detection of GFP fluorescence.

Paper Details

Date Published: 10 April 1998
PDF: 9 pages
Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); doi: 10.1117/12.307328
Show Author Affiliations
David J. Burns, Abbott Labs. (United States)
Elisabeth Alder, Abbott Labs. (United States)
Yi-Hong Fan, Abbott Labs. (United States)
Evelyn McKeegan, Abbott Labs. (United States)
Usha Warrior, Abbott Labs. (United States)
Bruce Beutel, Abbott Labs. (United States)

Published in SPIE Proceedings Vol. 3259:
Systems and Technologies for Clinical Diagnostics and Drug Discovery
Gerald E. Cohn; John C. Owicki, Editor(s)

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