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Proceedings Paper

Automated spectral imaging for clinical diagnostics
Author(s): John Breneman; David M. Heffelfinger; Ken Pettipiece; Chris Tsai; Peter Eden; Richard A. Greene; Karen J. Sorensen; Will Stubblebine; Frank Witney
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Paper Abstract

Bio-Rad Laboratories supplies imaging equipment for many applications in the life sciences. As part of our effort to offer more flexibility to the investigator, we are developing a microscope-based imaging spectrometer for the automated detection and analysis of either conventionally or fluorescently labeled samples. Immediate applications will include the use of fluorescence in situ hybridization (FISH) technology. The field of cytogenetics has benefited greatly from the increased sensitivity of FISH producing simplified analysis of complex chromosomal rearrangements. FISH methods for identification lends itself to automation more easily than the current cytogenetics industry standard of G- banding, however, the methods are complementary. Several technologies have been demonstrated successfully for analyzing the signals from labeled samples, including filter exchanging and interferometry. The detection system lends itself to other fluorescent applications including the display of labeled tissue sections, DNA chips, capillary electrophoresis or any other system using color as an event marker. Enhanced displays of conventionally stained specimens will also be possible.

Paper Details

Date Published: 10 April 1998
PDF: 9 pages
Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); doi: 10.1117/12.307320
Show Author Affiliations
John Breneman, Bio-Rad Labs. (United States)
David M. Heffelfinger, Bio-Rad Labs. (United States)
Ken Pettipiece, Bio-Rad Labs. (United States)
Chris Tsai, Bio-Rad Labs. (United States)
Peter Eden, Bio-Rad Labs. (United States)
Richard A. Greene, Bio-Rad Labs. (United States)
Karen J. Sorensen, Bio-Rad Labs. (United States)
Will Stubblebine, Bio-Rad Labs. (United States)
Frank Witney, Bio-Rad Labs. (United States)

Published in SPIE Proceedings Vol. 3259:
Systems and Technologies for Clinical Diagnostics and Drug Discovery
Gerald E. Cohn; John C. Owicki, Editor(s)

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