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Proceedings Paper

Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein
Author(s): SungHo Kim; Young Soon Noh; Gill Sang Byun; Sung-Keun Jang
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Paper Abstract

A simple and inexpensive quantitative detection of protein in one droplet solution was achieved by a portable diode-laser- based-optical-fiber absorption spectrometry. A system consisted of a visible diode laser, a photodiode and a pair of optical fibers. The conventional Lowry method was used to measure the blue-colored protein solutions. In this study, one droplet of sample, which filled up the void between the end surfaces of two optical fibers, served as a sample cell. The volume of a droplet was 50 (mu) L, which is 1/100 of the cuvette of UV/VIS spectrometer cell used in Lowry method. Although the required sample volume decreased up to 100 times, the detection range was comparable to that of conventional Lowry method in the range of g/L - mg/L.

Paper Details

Date Published: 29 April 1998
PDF: 6 pages
Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998);
Show Author Affiliations
SungHo Kim, Soonchunhyang Univ. (South Korea)
Young Soon Noh, Soonchunhyang Univ. (South Korea)
Gill Sang Byun, Soonchunhyang Univ. (South Korea)
Sung-Keun Jang, Soonchunhyang Univ. (South Korea)

Published in SPIE Proceedings Vol. 3260:
Optical Investigations of Cells In Vitro and In Vivo
Robert C. Leif; Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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