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Proceedings Paper

Determination of metal ions by fluorescence anisotropy exhibits a broad dynamic range
Author(s): Richard B. Thompson; Badri P. Maliwal; Carol A. Fierke
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Paper Abstract

Recently, we have shown that metal ions free in solution may be determined at low levels by fluorescence anisotropy (polarization) measurements. Anisotropy measurements enjoy the advantages of wavelength ratiometric techniques for determining metal ions such as calcium, because anisotropy measurements are ratiometric as well. Furthermore, fluorescence anisotropy may be imaged in the microscope. An advantage of anisotropy not demonstrated for wavelength ratiometric approaches using indicators such as Fura-2 and Indo-1 is that under favorable circumstances anisotropy-based determinations exhibit a much broader dynamic range in metal ion concentration. Determinations of free Zn(II) in the picomolar range are demonstrated.

Paper Details

Date Published: 1 May 1998
PDF: 9 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307077
Show Author Affiliations
Richard B. Thompson, Univ. of Maryland School of Medicine/Baltimore (United States)
Badri P. Maliwal, Univ. of Maryland School of Medicine/Baltimore (United States)
Carol A. Fierke, Duke Univ. Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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