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Proceedings Paper

Flow-cytometry-based DNA hybidization and polymorphism analysis
Author(s): Hong Cai; Kristina Kommander; P. Scott White; John P. Nolan
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Paper Abstract

Functional analysis of the human genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well- suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. We are developing a suite of microsphere-based approaches employing fluorescence detection to screen and analyze genomic sequence. Our approaches include competitive DNA hybridization to measure DNA or RNA targets in unknown samples, and oligo ligation or extension assays to analyze single-nucleotide polymorphisms. Apart from the advantages of sensitivity, simplicity, and low sample consumption, these flow cytometric approaches have the potential for high throughput multiplexed analysis using multicolored microspheres and automated sample handling.

Paper Details

Date Published: 1 May 1998
PDF: 7 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307060
Show Author Affiliations
Hong Cai, Los Alamos National Lab. (United States)
Kristina Kommander, Los Alamos National Lab. (United States)
P. Scott White, Los Alamos National Lab. (United States)
John P. Nolan, Los Alamos National Lab. (United States)

Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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