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Proceedings Paper

Probing biological systems with near-field optics
Author(s): Hitoshi Shiku; Christopher W. Hollars; M. Annie Lee; Chad E. Talley; Greg Cooksey; Robert C. Dunn
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Paper Abstract

The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images form a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.

Paper Details

Date Published: 24 April 1998
PDF: 9 pages
Proc. SPIE 3273, Laser Techniques for Condensed-Phase and Biological Systems, (24 April 1998); doi: 10.1117/12.306124
Show Author Affiliations
Hitoshi Shiku, Univ. of Kansas (United States)
Christopher W. Hollars, Univ. of Kansas (United States)
M. Annie Lee, Univ. of Kansas (United States)
Chad E. Talley, Univ. of Kansas (United States)
Greg Cooksey, Univ. of Kansas (United States)
Robert C. Dunn, Univ. of Kansas (United States)

Published in SPIE Proceedings Vol. 3273:
Laser Techniques for Condensed-Phase and Biological Systems
Norbert F. Scherer; Janice M. Hicks, Editor(s)

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