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Proceedings Paper

Determination of cell culture medium components with overlapping near-IR absorbance peaks
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Paper Abstract

A noninvasive method to measure levels of important tissue culture nutrients and metabolic by-products would provide the potential for computer control of the growth process, decreasing the amount of human attention necessary while improving efficiency and repeatability of experiments by precise maintenance of important parameters without contaminating the culture environment. Our approach to meeting this need is the development of optical sensor that extract chemical information based on the absorbance of near-IR radiation. Near-IR spectroscopy of the 2.0-2.5 micrometers region was used to collect absorbance data of aqueous mixtures containing glucose, lactate, ammonia, glutamate, and glutamine, as well as cell culture media samples from a three-day growth period. Multivariate calibration was used with these data to prove that complicated spectra can yield reasonable prediction errors. Calibration models using different combinations of spectra from aqueous mixtures and culture media were built and evaluated for glucose, ammonia, and lactate. Prediction errors of 2 percent, 8 percent, and 15 percent were obtained for glucose, lactate, and ammonia, respectively.

Paper Details

Date Published: 24 April 1998
PDF: 5 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306108
Show Author Affiliations
Michael J. McShane, Texas A&M Univ. (United States)
Gerard L. Cote, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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