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Proceedings Paper

Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro
Author(s): Luciana Almeida-Lopes D.D.S.; Marcia M. M. Jaeger; Aldo Brugnera Jr.; Josepa Rigau
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Paper Abstract

The low level power laser has been used in dental treatments aiming to improve tissue healing. An in vitro study was performed to analyze the laser influence on gingival fibroblast. A human gingival fibroblast culture (LMF) was produced in DME medium with 10% bovine fetal serum (BFS) cells (LMF) were allocated in Petri plates and cultured in different SFB concentrations (0%, 5% e 10%). After 48 hours the plates were divided in 9 groups: 3 control: 3 irradiated by 635 nm laser; and 3 irradiated by 780 nm laser. The cultured cells received 4 applications, in 12 hours intervals, with energy dosage of 2 joules for each plate, by means of a punctual technique. The growth curves showed that the growth levels were lower in low BFS concentrations. The irradiation with laser accelerated the growth rate in all groups. Additionally, the number of cells developed in low BFS concentration (5%) and irradiated was similar to the number of control cells developed in ideal conditions (10% BFS). There was no statistically significant differences between the effects of the two types of laser studied.

Paper Details

Date Published: 22 April 1998
PDF: 6 pages
Proc. SPIE 3248, Lasers in Dentistry IV, (22 April 1998); doi: 10.1117/12.306022
Show Author Affiliations
Luciana Almeida-Lopes D.D.S., Camilo Castelo Branco Univ. (Brazil)
Marcia M. M. Jaeger, Univ. de Sao Paulo (Brazil)
Aldo Brugnera Jr., Camilo Castelo Branco Univ. (Brazil)
Josepa Rigau, Univ. de Rovira i Virgili (Spain)

Published in SPIE Proceedings Vol. 3248:
Lasers in Dentistry IV
John D. B. Featherstone; Peter Rechmann D.D.S.; Daniel Fried, Editor(s)

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