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Proceedings Paper

Raman scattering spectra of insect virus PrGV-VP and their surface-enhanced Raman scattering spectra adsorbed on silver
Author(s): PeiDi Bao; TianQuan Huang; XinMing Liu; Tieqiao Wu
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Paper Abstract

The ordinary Raman scattering (ORS) spectra of virion protein of Granulosis Virus of Cabbage Butterfly Preris rapae (PrGV-VP) and their surface-enhanced Raman scattering (SERS) spectra adsorbed on the surface of silver colloid particles have been found that the Raman signal in the SERS spectra was enhanced by a factor of about 8 X 102. In ORS spectra of PrGV-VP, it indicates that PrGV-VP has a predominantly random-coil secondary structures. All tyrosine residues are exposed. And the PrGV-VP has a gauche-gauche- gauche configuration of the C-C-S-S-C-C linkage. In SERS spectra of PrGV-VP, the intensities of bands related to COO group and NH2 group vibrations are relatively strong. We interpret that the PrGV-VP are adsorbed on the Ag surface through both the carboxylate groups and the amino groups. The efficient enhancement of amide I and amide II vibrations are impeded, because the peptide groups of PrGV-VP are screened by the side-chains amino acid residues. The Raman enhancement of PrGV-VP has a short-range mechanism. The chemisorption is a main mechanism on Ag hydrosols of PrGV- VP.

Paper Details

Date Published: 16 April 1998
PDF: 6 pages
Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); doi: 10.1117/12.305384
Show Author Affiliations
PeiDi Bao, Sichuan Univ. (China)
TianQuan Huang, Sichuan Univ. (China)
XinMing Liu, Sichuan Univ. (China)
Tieqiao Wu, Sichuan Univ. (China)


Published in SPIE Proceedings Vol. 3250:
Optical Biopsy II
Robert R. Alfano, Editor(s)

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