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Proceedings Paper

Multiangle-multiwavelength UV/visible spectroscopy for the characterization of the joint property distribution of whole blood and its components
Author(s): Christina P. Bacon; Luis H. Garcia-Rubio
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Paper Abstract

The characterization of biological fluids for diagnostic purposes implies the estimation of the particle size distribution, the chemical composition, the shape distribution and the charge of the particles suspended in the fluid. These particle properties are defined as the joint particle property distribution. The shape distribution is especially important for the identification of healthy cells, sickle cells, viruses and bacteria. platelet viability can be determined from cell shape, discoid versus spheroid. Recent theoretical and simulations of the scattering behavior of particle dispersions using Mie and Rayleigh-Gans-Debye scattering models have demonstrated that complementary information on the joint property distribution is available from angular measurements of combined absorption and scattering spectra. A new multiangle- multiwavelength (MAMW) spectrophotometer was designed and developed using state-of-the-art fiber optic technology for the characterization of concentrated and dilute dispersion of biological fluids, primarily for whole blood and platelets. Miniature fiber optic UV/vis spectrometers were used to create a portable and robust characterization system; the spectrometer allows for the application of fields and easy adaptation for on-line analysis. This instrumentation was successfully tested for scattering measurement sensitivity and reproducibility and is capable of simultaneous absorption and scattering measurements at several angles and over a wide wavelength range. The MAMW spectrophotometer has been sued to analyze sizes of macromolecules and particle standards. Experimental results demonstrate that this technology can be used for the characterization of the joint property distribution of dilute dispersion of platelets, other biological particles and fluids.

Paper Details

Date Published: 16 April 1998
PDF: 9 pages
Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); doi: 10.1117/12.305371
Show Author Affiliations
Christina P. Bacon, Univ. of South Florida (United States)
Luis H. Garcia-Rubio, Univ. of South Florida (United States)

Published in SPIE Proceedings Vol. 3250:
Optical Biopsy II
Robert R. Alfano, Editor(s)

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