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Proceedings Paper

Implantation of microfabricated immunoisolating biocapsules
Author(s): Tejal A. Desai; Wen Hwa Chu; Mauro Ferrari; Guido Rasi; Paola Sinibaldi-Vallebona; Patrizia Borboni; G. Beattie; A. Hayek
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Paper Abstract

Silicon-based biocapsules have been microfabricated with uniform and well-controlled pore dimensions in the tens of nanometer range to provide effective immunoisolation of cell xenografts. Surface and bulk micromachining were integrated in the fabrication process, resulting in a diffusion membrane with mechanical and chemical stability, surrounded by an anisotropically-etched silicon wafer, which serves as the encapsulation cavity. The membrane allows the diffusion of essential nutrients while blocking the passage of immune molecules, which may destroy cellular transplants. Preliminary short term studies on both primary pancreatic islets and insulinoma cell lines encapsulated within microfabricated biocapsules were conducted to determine the toxicity and biocompatibility of biocapsules, the viability and functionality of encapsulated cells, as well as the overall immunoprotective capabilities of the biocapsule. Results seem to indicate that microfabricated biocapsules are non-toxic and do not elicit any adverse inflammatory reactions when implanted. Furthermore, encapsulated insulinoma cells remained viable and functional within microfabricated environments in vivo. These results show the feasibility and potential application of microfabricated biocapsules for several pathologies.

Paper Details

Date Published: 26 March 1998
PDF: 8 pages
Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304387
Show Author Affiliations
Tejal A. Desai, Univ. of California/Berkeley (United States)
Wen Hwa Chu, Univ. of California/Berkeley (United States)
Mauro Ferrari, Univ. of California/Berkeley (United States)
Guido Rasi, Istituto di Medicina Sperimentale (Italy)
Paola Sinibaldi-Vallebona, Istituto di Medicina Sperimentale (Italy)
Patrizia Borboni, Istituto di Medicina Sperimentale (Italy)
G. Beattie, Univ. of California/San Diego (United States)
A. Hayek, Univ. of California/San Diego (United States)

Published in SPIE Proceedings Vol. 3258:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications
Paul Lee Gourley, Editor(s)

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