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Proceedings Paper

Surface-plasmon-resonance-based array biosensors for multianalyte detection
Author(s): Michael J. O'Brien II; Victor H. Perez-Luna; Leonard M. Tender; Mark Edmunds; Ben Lascelles; Steven R. J. Brueck; Gabriel P. Lopez
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Paper Abstract

Surface plasmon resonance (SPR) is a phenomenon wherein the reflectance versus angle-of-incidence profile for a thin gold film illuminated with p-polarized light has a distinct minimum at a particular angle. This minimum of reflectance is due to an absorption of the light energy by the surface electron plasma of the metal occurring when the surface- parallel components of the light and plasmon propagation vectors match up. The value of this particular angle of incidence changes in proportion to the degree of adsorption of analytes to the metal film. This allows SPR to be used as a simple, noninvasive, optical tool for measuring the binding of chemical analytes. With a predetermined pattern of chemically specific receptors bound to the gold film, it is possible to detect a variety of species and concentrations of analytes, provided that one has a sensor platform capable of resolving the different reactions in each element of the receptor array. We have developed such a platform which is capable of optically monitoring an array of analyte receptors immobilized on gold coated microscope slides in real time. Moreover, the optical resolution of sensor platform allows the receptors to be micro-patterned.

Paper Details

Date Published: 26 March 1998
PDF: 11 pages
Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304386
Show Author Affiliations
Michael J. O'Brien II, Univ. of New Mexico (United States)
Victor H. Perez-Luna, Univ. of New Mexico (United States)
Leonard M. Tender, Univ. of New Mexico (United States)
Mark Edmunds, Univ. of New Mexico (United States)
Ben Lascelles, Univ. of New Mexico (United States)
Steven R. J. Brueck, Univ. of New Mexico (United States)
Gabriel P. Lopez, Univ. of New Mexico (United States)

Published in SPIE Proceedings Vol. 3258:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications
Paul Lee Gourley, Editor(s)

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