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Proceedings Paper

Low-coherence in-depth microscopy for biological tissue imaging: design of a real-time control system
Author(s): Loic Blanchot; Martial Lebec; Emmanuel Beaurepaire; Philippe Gleyzes; Albert Claude Boccara; Herve Saint-Jalmes
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Paper Abstract

We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock- in detection method that requires accurate synchronization of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 micrometers , and the coherence length of the light source defines an axial resolution of approximately 8 micrometers . Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.

Paper Details

Date Published: 1 January 1998
PDF: 7 pages
Proc. SPIE 3194, Photon Propagation in Tissues III, (1 January 1998); doi: 10.1117/12.301057
Show Author Affiliations
Loic Blanchot, Univ. Paris XII (France)
Martial Lebec, Univ. Paris XII (France)
Emmanuel Beaurepaire, Ecole Superieure de Physique et de Chimie Industrielles (France)
Philippe Gleyzes, Ecole Superieure de Physique et de Chimie Industrielles (France)
Albert Claude Boccara, Ecole Suereieure de Physique et de Chimie Industrielles (France)
Herve Saint-Jalmes, Univ. Paris XII (France)

Published in SPIE Proceedings Vol. 3194:
Photon Propagation in Tissues III
David A. Benaron M.D.; Britton Chance; Marco Ferrari, Editor(s)

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