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Proceedings Paper

Analysis of human colon tissue using Raman spectroscopy
Author(s): Sarah E. Cope; Tami L. Freeman; B. J. Rembacken; Mark R. Stringer; David N. Batchelder; Stanley B. Brown
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Paper Abstract

Near-infrared Raman microscopy was used to study normal, adenomatous and carcinomatous colon tissue in vitro. Samples were fixed in formalin and it was shown that this procedure did not alter the observed Raman peaks of the tissue, and is therefore a suitable method of fixation. The samples were observed visually to be of varying shades ranging from cream to dark brown; this was independent of whether the samples were cancerous or normal. Differences were seen between the Raman spectra of the different colored tissue with the spectra of brown tissue displaying a greater number of resolved peaks. We believe these differences may be due to differing amounts of blood present in the tissue. This must be considered when using Raman spectra for diagnostic work to ensure that differences ascribed to cancerous changes are not in fact due to differing amounts of blood within the sample.

Paper Details

Date Published: 29 December 1997
PDF: 5 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297982
Show Author Affiliations
Sarah E. Cope, Research School of Medicine/Univ. of Leeds (United Kingdom)
Tami L. Freeman, Research School of Medicine/Univ. of Leeds (United Kingdom)
B. J. Rembacken, Leeds General Infirmary (United Kingdom)
Mark R. Stringer, Univ. of Leeds (United Kingdom)
David N. Batchelder, Univ. of Leeds (United Kingdom)
Stanley B. Brown, Univ. of Leeds (United Kingdom)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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