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Proceedings Paper

FTIR microspectroscopic imaging of human carcinoma thin tissue sections
Author(s): Peter Lasch; Wolfgang Waesche; Uwe Bindig; Dieter Naumann; Gerhard J. Mueller
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Paper Abstract

The FT-IR-microspectroscopic mapping technique in combination with image construction methods has been used to characterize thin tissue sections from human melanoma. IR mapping spectroscopy as a spatially two-dimensional working technique is a non-invasive analytical method. Up to now IR-imaging has been based on distinct spectral parameters such as frequency, intensity or fullwidth of half maximum, ratio of single wavelengths and so on. We decided to compare this technique with other chemometric methods and to secure that these parameters will not give unsatisfactory results. For this purpose pattern recognition analysis (PRA) e.g. principal component analysis (PCA) or artificial neural networks (ANN) of IR-data, confirmed with standard histopathological techniques, has turned out to be helpful to discriminate also between different tissues.

Paper Details

Date Published: 29 December 1997
PDF: 8 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297974
Show Author Affiliations
Peter Lasch, Robert Kock-Institut (Germany)
Wolfgang Waesche, Laser- und Medizin Technologie gGmbH (Germany)
Uwe Bindig, Universitatsklinikum Benjamin Franklin der Freien Univ. Berlin (Germany)
Dieter Naumann, Robert Koch-Institut (Germany)
Gerhard J. Mueller, Universitatsklinikum Benjamin Franklin der Freien Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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