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Proceedings Paper

Time-resolved energy transfer spectroscopy for measuring mitochondrial metabolism in living cells
Author(s): Herbert Schneckenburger; Michael H. Gschwend; Wolfgang S. L. Strauss; Reinhard Sailer; Manfred Bauer; Rudolf W. Steiner
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Paper Abstract

Energy transfer from NADH to the mitochondrial marker rhodamine 123 (R123) was used to probe mitochondrial malfunction of cultivated endothelial cells incubated with various inhibitors of specific enzyme complexes of the respiratory chain. Pronounced differences of 'energy transfer efficacy' of incubated cells as compared to controls were deduced from the ratio of fluorescence intensity and intracellular amount of the acceptor. A combination of cw and time-gated (nanosecond) fluorescence spectroscopy appeared to be an appropriate tool for probing mitochondrial malfunction in various kinds of diseases.

Paper Details

Date Published: 29 December 1997
PDF: 8 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297964
Show Author Affiliations
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)
Reinhard Sailer, Univ. Ulm (Germany)
Manfred Bauer, Univ. Ulm (Germany)
Rudolf W. Steiner, Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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