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Proceedings Paper

Different PS application methods for laserlight-induced fluorescence diagnostics (LIFD) of dysplasia and early cancer of the colon
Author(s): Johannes Gahlen; Jochen Pressmar; Josef Stern; J. Boehm; Hanns-joerg Sinn; I. A. Born; R. Holle; Christian Herfarth
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Paper Abstract

Conventional diagnostics fail to trace dysplasias and early cancers of the colon. By means of Laserlight Induced Fluorescence Diagnostics (LIFD) macroscopically not visible malignant tumors and dysplasias of the colon can be detected. The main side effect of systemically applicated photosensitizers is a general photosensibilization of the organism which provokes an increased photosensitivity of the body. Local application of (delta) -aminolevulinic acid (ALA) reduces systemic uptake without impairment of the quality of investigation. Colorectal cancer in male wistar rats was induced by weekly subcutaneous (s.c.) injections of 1,2- dimethylhydrazine (DMH) for 17 weeks. After s.c. DMH injection the wistar rats (n equals 25) developed in average 5.6 macroscopically visible colon carcinomas. 80% of these tumor were detected by LIFD after photosensibilization with Photofrin II intravenously (i.v.), 29% after application of mTHPC (i.v.), 13% after ALA (i.v.), 20% after intraperitoneal application of ALA (i.p.) and 57% after local application of ALA in the colon. LIFD is a capable method for the detection of macroscopically not visible dysplasias and colon carcinomas of the rat induced by DMH. The applied PS and the method of application influences the detection of carcinomas and dysplasias. Systemic photosensibilization of the whole organism can be avoided by the local application of ALA without limiting the quality of investigation.

Paper Details

Date Published: 22 December 1997
PDF: 6 pages
Proc. SPIE 3192, Medical Applications of Lasers in Dermatology, Ophthalmology, Dentistry, and Endoscopy, (22 December 1997);
Show Author Affiliations
Johannes Gahlen, Chirurgische Universitaetsklinik Heidelberg (Germany)
Jochen Pressmar, Chirurgische Universitaetsklinik Heidelberg (Germany)
Josef Stern, Chirurgische Universitaetsklinik Heidelberg (Germany)
J. Boehm, Chirurgische Universitaetsklinik Heidelberg (Germany)
Hanns-joerg Sinn, Deutsches Krebsforschungszentrum (Germany)
I. A. Born, Pathologisches Institut (Germany)
R. Holle, GSF Forschungszentrum (Germany)
Christian Herfarth, Chirurgische Universitaetsklinik Heidelberg (Germany)

Published in SPIE Proceedings Vol. 3192:
Medical Applications of Lasers in Dermatology, Ophthalmology, Dentistry, and Endoscopy
Gregory B. Altshuler; Reginald Birngruber; Marco Dal Fante; Raimund Hibst; Herbert Hoenigsmann; Neville Krasner M.D.; Frederic Laffitte, Editor(s)

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