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Proceedings Paper

Photodynamic nerve cell killing: dynamics of electrophysiological responses and comparison of photosensitizers
Author(s): Anatoly B. Uzdensky
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Paper Abstract

The study of single neuron response to photodynamic effect provides evaluation of the dynamics of cytotoxic events leading from initial threshold changes to the cell death and allows us to compare phototoxicity of different substances. The photosensitized isolated crayfish mechanoreceptor neurons were irradiated with the He-Ne laser (632.8 nm, 0.3 W/cm2), and their responses were recorded. The following photosensitizers were studied: methylene blue, janus green B, protoporphyrin IX, chlorins e6 and p6 and sulphonated aluminum phthalocyanine 'Photosens.' Crayfish neurons were insensitive to the He-Ne laser irradiation and photosensitization alone, but very sensitive to photodynamic effect: they changed firing rate and died at nanomolar concentrations of photosensitizers. The dynamics of neuron responses depended on the photosensitizer type and its concentration. Dependencies of firing acceleration rate and neuron lifetime on photosensitizer concentration allowed to compare efficiencies of different photosensitizers. Photosens and chlorin p6 were found to be the most potent photosensitizers.

Paper Details

Date Published: 29 December 1997
PDF: 10 pages
Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); doi: 10.1117/12.297794
Show Author Affiliations
Anatoly B. Uzdensky, Rostov State Univ. (Russia)

Published in SPIE Proceedings Vol. 3191:
Photochemotherapy: Photodynamic Therapy and Other Modalities III
Kristian Berg; Benjamin Ehrenberg; Zvi Malik; Johan Moan; Abraham Katzir, Editor(s)

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