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Proceedings Paper

X-ray irradiation of yeast cells
Author(s): Alessandra Masini; Dimitri Batani; Fabio Previdi; Aldo Conti; Francesca Pisani; Cesare Botto; Fulvia Bortolotto; Flavia Torsiello; I. C. Edmond Turcu; Ric M. Allott; Nicola Lisi; Marziale Milani; Michele Costato; Achille Pozzi; Michel Koenig
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Paper Abstract

Saccharomyces Cerevisiae yeast cells were irradiated using the soft X-ray laser-plasma source at Rutherford Laboratory. The aim was to produce a selective damage of enzyme metabolic activity at the wall and membrane level (responsible for fermentation) without interfering with respiration (taking place in mitochondria) and with nuclear and DNA activity. The source was calibrated by PIN diodes and X-ray spectrometers. Teflon stripes were chosen as targets for the UV laser, emitting X-rays at about 0.9 keV, characterized by a very large decay exponent in biological matter. X-ray doses to the different cell compartments were calculated following a Lambert-Bouguet-Beer law. After irradiation, the selective damage to metabolic activity at the membrane level was measured by monitoring CO2 production with pressure silicon detectors. Preliminary results gave evidence of pressure reduction for irradiated samples and non-linear response to doses. Also metabolic oscillations were evidenced in cell suspensions and it was shown that X-ray irradiation changed the oscillation frequency.

Paper Details

Date Published: 14 October 1997
PDF: 15 pages
Proc. SPIE 3157, Applications of X Rays Generated from Lasers and Other Bright Sources, (14 October 1997); doi: 10.1117/12.283997
Show Author Affiliations
Alessandra Masini, Univ. di Milano (Italy) and INFM (Italy)
Dimitri Batani, Univ. di Milano (Italy) and INFM (Italy)
Fabio Previdi, Univ. di Milano (Italy) and INFM (Italy)
Aldo Conti, Univ. di Milano (Italy) and INFM (Italy)
Francesca Pisani, Univ. di Milano (Italy) and INFM (Italy)
Cesare Botto, Univ. di Milano (Italy) and INFM (Italy)
Fulvia Bortolotto, Univ. di Milano (Italy) and INFM (Italy)
Flavia Torsiello, Univ. di Milano (Italy) and INFM (Italy)
I. C. Edmond Turcu, Rutherford Appleton Lab. (United Kingdom)
Ric M. Allott, Rutherford Appleton Lab. (United Kingdom)
Nicola Lisi, Rutherford Appleton Lab. (United Kingdom)
Marziale Milani, Univ. di Milano (Italy)
Michele Costato, Univ. di Modena (Italy)
Achille Pozzi, Univ. di Modena (Italy)
Michel Koenig, Ecole Polytechnique (France)

Published in SPIE Proceedings Vol. 3157:
Applications of X Rays Generated from Lasers and Other Bright Sources
George A. Kyrala; Jean-Claude J. Gauthier, Editor(s)

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