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Proceedings Paper

Biochemical distinctions between normal and cancerous human breast tissues obtained from fluorescence spectroscopy
Author(s): Niclolay N. Zhadin; Yuanlong Yang; Nathan Ockman; Robert R. Alfano
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Paper Abstract

A novel method for correcting the fluorescence emission and excitation spectra is applied to native fluorescence spectra from normal and cancerous human breast tissues. The method effectively eliminates the distortions produced by internal light-absorption and allows a direct, real-time, correction without any iterative procedures. A simplified photon- diffusion model was used to develop the method. An analysis of both the true fluorescence spectra, and the diffuse reflectance spectra transformed into the ratio of absorption and reduced scattering coefficients, shows distinctive biochemical differences between cancerous and normal breast tissues. The fluorescence spectra feature a lower contribution of NADH and, possibly, collagen and elastin in cancerous tumor tissues as compared with normal tissues. The fluorescence spectra from cancerous tumors also show a lower degree of variability than the spectra from normal tissues. The corrected spectra from cancerous tumors show a greater similarity in their profiles than the non-corrected fluorescence spectra distorted by the internal light- absorption.

Paper Details

Date Published: 18 August 1997
PDF: 4 pages
Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); doi: 10.1117/12.280294
Show Author Affiliations
Niclolay N. Zhadin, CUNY/City College (United States)
Yuanlong Yang, CUNY/City College (United States)
Nathan Ockman, CUNY/City College (United States)
Robert R. Alfano, CUNY/City College (United States)

Published in SPIE Proceedings Vol. 2979:
Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II
Britton Chance; Robert R. Alfano, Editor(s)

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