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Proceedings Paper

Optical biosensing using surface plasmon resonance spectroscopy
Author(s): Jiri Homola; Peter Pfeifer; Eduard Brynda; Jiri Skvor; Milan Houska; Guenter Schwotzer; Ines Latka; Reinhardt Willsch
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Paper Abstract

The exploitation of surface plasmon resonance (SPR) sensing principle for the study of formation of multilayers of human immunoglobulin (hlgG) and monoclonal antibody against human choriogonadotropin (a-hCG) as well as for monitoring immunoreaction between the immobilized a-hCG and human choriogonadotropin (hCG) is reported. It is demonstrated that even a very small attachment of proteins (due to the adsorption or the specific interaction) may be detected by the presented SPR sensor. An immobilization technique is described which allows to form stable multilayers of antibodies on the gold surface.

Paper Details

Date Published: 30 May 1997
PDF: 7 pages
Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997);
Show Author Affiliations
Jiri Homola, Institute of Radio Engineering and Electronics (United States)
Peter Pfeifer, Institute of Physical High-Technology (Germany)
Eduard Brynda, Institute of Macromolecular Chemistry (Czech Republic)
Jiri Skvor, SEVA IMUNO Ltd. (Czech Republic)
Milan Houska, Institute of Macromolecular Chemistry (Czech Republic)
Guenter Schwotzer, Institute of Physical High-Technology (Germany)
Ines Latka, Institute of Physical High-Technology (Germany)
Reinhardt Willsch, Institute of Physical High-Technology (Germany)

Published in SPIE Proceedings Vol. 3105:
Chemical, Biochemical and Environmental Fiber Sensors IX
Robert A. Lieberman, Editor(s)

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