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Proceedings Paper

Biomedical diagnostic with diffusely backscattered linearly and circularly polarized light
Author(s): Andreas H. Hielscher; Angelia A. Eick; Judith R. Mourant; James P. Freyer; Irving J. Bigio
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Paper Abstract

We report on the development of a method that records spatially dependent intensity patterns of polarized light that is diffusely backscattered from turbid materials. It is demonstrated that these intensity patterns can be used to differentiate between suspensions of cancerous and non- cancerous cells and to observe different metabolic cell processes.Our technique employs polarized light from a He-Ne laser, which is focused onto the surface of the scattering medium. A surface area of approximately 4 X 4 cm around the light input point is imaged through polarization- analysis optics onto a CCD camera. One can observe a manifold of intensity patterns by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Introducing the Mueller-matrix concept for diffusely backscattered light, a framework is provided to select a subset of measurements which comprehensively describe the optical properties of backscattering media.

Paper Details

Date Published: 16 June 1997
PDF: 8 pages
Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); doi: 10.1117/12.275538
Show Author Affiliations
Andreas H. Hielscher, Los Alamos National Lab. (United States)
Angelia A. Eick, Los Alamos National Lab. (United States)
Judith R. Mourant, Los Alamos National Lab. (United States)
James P. Freyer, Los Alamos National Lab. (United States)
Irving J. Bigio, Los Alamos National Lab. (United States)

Published in SPIE Proceedings Vol. 2976:
Biomedical Sensing, Imaging, and Tracking Technologies II
Tuan Vo-Dinh; Robert A. Lieberman; Gerald G. Vurek; Abraham Katzir, Editor(s)

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