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Proceedings Paper

Effects of hydration on laser soldering
Author(s): Eric K. Chan; Dennis T. Brown; Ian S. Kovach; Ashley J. Welch
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Paper Abstract

Laser welding with albumin-based tissue solder has been investigated as an alternative to surgical suturing. Many surgical procedures require the soldered tissues to be in a hydrated environment. We have studied the effects of hydration on laser soldered rat dermis and baboon articular cartilage in vitro. The solder is composed of human serum albumin, sodium hyaluronate and indocyanine green. We used a micro-pipette to deposit 2 (mu) l of solder on each tissue specimen. An 808 nm cw laser beam with irradiance of 27 W/cm2 was scanned 4 times over the same solder area at a constant speed of 0.84 mm/sec. After photo-coagulation, each tissue specimen was cut into two halves at the center of the solder, perpendicular to the direction of the scanning laser beam. One half was reserved as control while the other half was soaked in phosphate buffered saline for a designated hydration period. The hydration periods were 1 hr, 1, 2, and 7 days. All tissue specimens were fixed in glutaraldahyde, then prepared for scanning electron microcopy analysis. For most of the specimens, there was non-uniform coagulation across the thickness of the solder. Closer to the laser beam, the upper solder region formed a more dense coagulum. While the region closer to solder-tissue interface, the solder aggregated into small globules. This non-uniform coagulation was likely caused by non-uniform energy distribution during photocoagulation. The protein globules and coagulum seem to be responsible for the solder attachment from the specimen surface. However, we have noted that the solder detached from the cartilage substrate as early as after 1 hr of hydration. On the other hand, the solder attached to the dermis much better than to cartilage. This may be explained by the difference in surface roughness of the two tissue types. The dermal layer of the skin is composed of collagen matrix which may provide a better entrapment of the solder than the smooth surface of articular cartilage.

Paper Details

Date Published: 22 May 1997
PDF: 8 pages
Proc. SPIE 2970, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII, (22 May 1997); doi: 10.1117/12.275049
Show Author Affiliations
Eric K. Chan, Univ. of Texas0/Austin (United States)
Dennis T. Brown, Univ. of Texas/Austin (United States)
Ian S. Kovach, Univ. of Texas/San Antonio Health Sciences Ctr. (United States)
Ashley J. Welch, Univ. of Texas/Austin (United States)

Published in SPIE Proceedings Vol. 2970:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII
R. Rox Anderson M.D.; Harvey Lui M.D.; Michail M. Pankratov; Kenneth Eugene Bartels D.V.M.; Gerhard J. Mueller; Graham M. Watson M.D.; Reza S. Malek M.D.; Lawrence S. Bass M.D.; Lloyd P. Tate V.D.M.; Hans-Dieter Reidenbach; Kenneth Eugene Bartels D.V.M.; R. Rox Anderson M.D.; Lawrence S. Bass M.D.; Aaron P. Perlmutter M.D.; Kenton W. Gregory M.D.; David M. Harris; David M. Harris; Harvey Lui M.D.; Reza S. Malek M.D.; Gerhard J. Mueller; Michail M. Pankratov; Aaron P. Perlmutter M.D.; Hans-Dieter Reidenbach; Lloyd P. Tate V.D.M.; Graham M. Watson M.D., Editor(s)

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