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Proceedings Paper

Wide-field imaging design for a multiple-capillary DNA-sequencing system
Author(s): Lyle M. Nay; Robert Sinclair; Harold Swerdlow
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Paper Abstract

A laser-induced fluorescence detection system compatible with a capillary electrophoresis array was developed. The design incorporates fiber-optic excitation and a detection system including a diffraction grating and a CCD camera. The system employs no moving parts and is capable of producing data comparable to commercially available systems. It is based on a spectrally-resolved four-dye sequencing scheme. The conceptual design was proven, however, refinements must be made to optimize performance for high-throughput capillary-array DNA sequencing. Automated sample preparation and loading in combination with a refillable separation- matrix capillary-array system could prove to be an invaluable tool for completion of the Human Genome Project.

Paper Details

Date Published: 22 May 1997
PDF: 6 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274354
Show Author Affiliations
Lyle M. Nay, Univ. of Utah (United States)
Robert Sinclair, Univ. of Utah (United States)
Harold Swerdlow, Univ. of Utah (United States)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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