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Proceedings Paper

Factors to be considered for robust high-throughput automated DNA sequencing using a multiple-capillary array instrument
Author(s): Emanuel Carrilho; Arthur W. Miller; Marie C. Ruiz-Martinez; Lev Kotler; Jeffrey Kesilman; Barry L. Karger
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Paper Abstract

The overall goal of our program is to develop a robust, high throughput, fully automated DNA sequencing instrument based on replaceable polymer solutions using a multicapillary array. Significant effort has already been devoted to column and polymer chemistry in order to obtain long read lengths per run in fast analysis time. In this paper we report on progress in instrument considerations and data processing software. A simple instrument design, based on no moving parts for continuous illumination of the capillaries and detection of the fluorescent light was used for this study. Our polymer solution replacement system with the permanent connection between the buffer/chamber manifold and capillary columns on the detector side is designed to prevent the trapping of air bubbles during matrix solution replacement. A special construction of a column-electrode couple on the injection side precludes air trapping during sample injection from small sample volumes. Our in-house software now features the significant reduction of the crosstalk signal from neighbor columns, which may be a potential problem in densely packed large capillary array sequencers.

Paper Details

Date Published: 22 May 1997
PDF: 15 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274344
Show Author Affiliations
Emanuel Carrilho, Northeastern Univ. and Univ. of Sao Paulo (Brazil)
Arthur W. Miller, Northeastern Univ. (United States)
Marie C. Ruiz-Martinez, Northeastern Univ. (United States)
Lev Kotler, Northeastern Univ. (United States)
Jeffrey Kesilman, Northeastern Univ. (United States)
Barry L. Karger, Northeastern Univ. (United States)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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