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Proceedings Paper

Neurotransmitter measurement with a fiber optic probe using pulsed ultraviolet resonance Raman spectroscopy
Author(s): H. Georg Schulze; L. Shane Greek; Michael W. Blades; Alan V. Bree; Boris B. Gorzalka; Robin F. B. Turner
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Paper Abstract

Many techniques have been developed to investigate the chemistry associated with brain activity. These techniques generally fall into two categories: fast techniques with species restricted sensitivity and slow techniques with generally unrestricted species sensitivity. Therefore, a need exists for a fast non-invasive technique sensitive to a wide array of biologically relevant compounds in order to measure chemical brain events in real time. The work presented here describes the progress made toward the development of a novel neurotransmitter probe. A fiber-optic linked Raman and tunable ultraviolet resonance Raman system was assembled with custom designed optical fiber probes. Probes of several different geometries were constructed and their working curves obtained in aqueous mixtures of methyl orange and potassium nitrate to determine the best probe configuration given particular sample characteristics. Using this system, the ultraviolet resonance Raman spectra of some neurotransmitters were measured with a fiber-optic probe and are reported here for the first time. The probe has also been used to measure neurotransmitter secretions obtained from depolarized rat pheochromocytoma cells.

Paper Details

Date Published: 2 May 1997
PDF: 9 pages
Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); doi: 10.1117/12.273613
Show Author Affiliations
H. Georg Schulze, Univ. of British Columbia (Canada)
L. Shane Greek, Univ. of British Columbia (Canada)
Michael W. Blades, Univ. of British Columbia (Canada)
Alan V. Bree, Univ. of British Columbia (Canada)
Boris B. Gorzalka, Univ. of British Columbia (Canada)
Robin F. B. Turner, Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 2982:
Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology
Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Robert C. Leif, Editor(s)

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