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Proceedings Paper

Spectroscopic evaluation of polyazamacrocyclic Tb(III) chelates for application in diagnostic imaging
Author(s): Darren S. Hubbard; Michael P. Houlne; Garry Kiefer; Darryl J. Bornhop
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Paper Abstract

The use of fluorescence probes for in-vivo diagnostics is at the forefront of medical science. To transition this technique into the clinical environment, quantitative spectral analysis and knowledge of the cellular interactions of the marker probes is vital. Furthermore, fluorescence intensity and lifetime changes, as a function of physiological environment, represents a diagnostic opportunity. A new class of polyazamacrocyclic chelates of Terbium have been identified with rich spectroscopic properties. These chelates are tissue selective, have fluorescence lifetimes on the order of milliseconds, sharply spiked emission spectra (< 15 nm FWHM), large Stokes shifts (> 280 nm), good water solubility and high quantum yields (approximately 0.6 for PCTMB). We will present our in-vitro and in-vivo spectroscopic evaluation of the chelates. In addition to the spectral investigations, results from cellular binding specificity studies using Sprague-Dawley rats with UMR 108 osteosarcomas will be presented. The potential to use the Tb(III) chelates as neoplastic tissue markers will be discussed.

Paper Details

Date Published: 7 May 1997
PDF: 7 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273572
Show Author Affiliations
Darren S. Hubbard, Texas Tech Univ. (United States)
Michael P. Houlne, Texas Tech Univ. (United States)
Garry Kiefer, Dow Chemical Co. (United States)
Darryl J. Bornhop, Texas Tech Univ. (United States)

Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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