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Proceedings Paper

Hydrodynamic activation and sorting of white blood cells in a microfabricated lattice
Author(s): Robert H. Carlson; Christopher V. Gabel; Shirley S. Chan; Robert H. Austin; James P. Brody; James W. Winkelman M.D.
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Paper Abstract

We demonstrate a novel hydrodynamic shear activation of leucocyte adhesion, using physiological flow conditions and a microfabricated array of channels with length scales similar to those of human capillaries. Vital chromosome stains and cell specific fluorochrome labeled antibodies reveal that the eventual adhesion of the leukocytes to the silicon array displays a strong dependence on cell type and nuclear morphology, with granulocytes activating more rapidly with distance and penetrating a smaller distance than lymphocytes. Further, the granulocytes interact with the lymphocytes in a self-exclusionary manner under shearing flow with the eventual separation of the two cell types in the array. Such arrays of microfabricated obstacles thus have an interesting potential for sorting white blood cells by type from a 10 microliter drop of whole blood.

Paper Details

Date Published: 31 March 1997
PDF: 10 pages
Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); doi: 10.1117/12.269970
Show Author Affiliations
Robert H. Carlson, Princeton Univ. (United States)
Christopher V. Gabel, Princeton Univ. (United States)
Shirley S. Chan, Princeton Univ. (United States)
Robert H. Austin, Princeton Univ. (United States)
James P. Brody, Univ. of Washington (United States)
James W. Winkelman M.D., Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 2978:
Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications
Paul Lee Gourley, Editor(s)

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