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Proceedings Paper

Fluorescence lifetime imaging of cells on the picosecond timescale
Author(s): Klaus Kemnitz; Rene Paul; Jacques Coppey; Maite Coppey-Moisan
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Paper Abstract

Novel MCP detectors for time- and space-correlated single photon counting (TSCSPC) spectroscopy, featuring delay-line (DL) or quadrant anode (QA), are employed in microscopic fluorescence lifetime imaging on the picosecond time scale. The linear DL-MCP-PMT is characterized by a spatial instrument response function (IRF) of 100 micrometer FWHM, resulting in 200 space channels, whereas the QA-MCP-PMT is a 2D imager with 400 by 400 pixel at 40 micrometer resolution. The detectors have a temporal IRF of 75 ps (DL) and 120 ps (QA) FWHM, sufficient for 10 ps time resolution. First results on TOTO-fixed cell systems are presented, demonstrating high-quality kinetics at subcellular resolution, with up to 6 lifetime species at higher dye concentrations, characteristically distributed among individual cell compartments. A comparison with TOTO/DNA- suspensions is made that serve as reference system.

Paper Details

Date Published: 6 December 1996
PDF: 11 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260795
Show Author Affiliations
Klaus Kemnitz, EuroPhoton GmbH (Germany)
Rene Paul, LaserLabor Adlershof GmbH (Germany)
Jacques Coppey, Institut Curie (France)
Maite Coppey-Moisan, Institut Curie (France)

Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest M.D.; Herbert Schneckenburger; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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