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Proceedings Paper

Fiber optic approach to the fluorescence spectroscopy of cell cultures
Author(s): Wolfgang Hoehne; Werner Schramm; V. Moritzen; U. Burgmann; Heinz Detlef Kronfeldt
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Paper Abstract

A fiber-optical spectrometer for pharmacologically oriented energy metabolism studies using NADH fluorescence has been built up. Starting from theoretical considerations, the system parameters have been optimized. With this optimized geometry, the sensitivity limit for quantitative NADH detection has reached 20 (mu) M. The absolute lower detectable concentration value limited by SNR and other background effects lies below 5 - 10 (mu) M. With cultures of endothelial cells, measurements of the age dependence of NADH fluorescence intensity have been performed. They give a criterion for the metabolic activity of cells selected for further pharmacological investigations.

Paper Details

Date Published: 11 December 1996
PDF: 7 pages
Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); doi: 10.1117/12.260633
Show Author Affiliations
Wolfgang Hoehne, Institute of Molecular Pharmacology and Technical Univ. Berlin (Germany)
Werner Schramm, Institute of Molecular Pharmacology (Germany)
V. Moritzen, Technical Univ. Berlin (Germany)
U. Burgmann, Technical Univ. Berlin (Germany)
Heinz Detlef Kronfeldt, Technical Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 2927:
Optical and Imaging Techniques for Biomonitoring II
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska M.D., Editor(s)

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